leptin Active Protein | lep active protein
Salamander leptin
Gene Names
lep; ob; xlep
Purity
The purity of recombinant salamander leptin is greater than 99.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) RP-chromatography
Synonyms
leptin; Salamander leptin; lep active protein
Host
E Coli
Purity/Purification
The purity of recombinant salamander leptin is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) RP-chromatography
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) RP-chromatography
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first ten N-terminal amino acids of recombinant salamander leptin was determined and was found to be Ala-Ile-Met-Val-Asp-Gln-Leu-Arg-Met-Asp.
Sequence Length
169
Solubility
It is recommended to reconstitute the lyophilized recombinant salamander leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml. Recombinant salamander leptin, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content
Protein quantitation of recombinant salamander leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.104 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified Sal-LEP (16K) consists of > 99% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.05 ng/ug (IEU/ug) of recombinant salamander leptin
Formulation
The recombinant salamander leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
recombinant salamander leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The activity of recombinant salamander leptin in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128 -136).
Preparation and Storage
Lyophilized recombinant salamander leptin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant salamander leptin at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant salamander leptin can be stored at +4 degree C for at least two weeks.
Related Product Information for lep active protein
Recombinant salamander leptin (Sal-LEP)is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kD. Recombinant salamander leptin was purified by proprietary chromatographic techniques, similarly to that described previously by Salomon et al (2006) Protein Expression and Purifcation 47, 128 -136.
Product Categories/Family for lep active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin<
NCBI Official Symbol
lep
NCBI Official Synonym Symbols
ob; xlep
NCBI Protein Information
leptin; obesity homolog
Protein Family
Research Articles on lep
Similar Products
Product Notes
The lep (Catalog #AAA400096) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first ten N-terminal amino acids of recombinan t salamander leptin was determined and was found to be Ala-Ile-Me t-Val-Asp- Gln-Leu-Ar g-Met-Asp. It is sometimes possible for the material contained within the vial of "leptin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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