Leptin Active Protein | Lep active protein

Recombinant Mouse Leptin

Cat. Num. AAA142228

• Gene Names: Lep; ob; obese
• Purity: Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Leptin; Recombinant Mouse Leptin; Leptin Mouse; Leptin Mouse Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; mLeptin; Lep active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
• Sequence Length: 167

• Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
• Biological Activity: Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
• Preparation and Storage: Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for Lep active protein

Description: Leptin Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.The Leptin is purified by proprietary chromatographic techniques.

Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

Product Categories/Family for Lep active protein

Cytokines and Growth Factors; Growth Factors; Leptin

NCBI and Uniprot Product Information

• NCBI GI #: 6678678
• NCBI GeneID: 16846
• NCBI Accession #: NP_032519.1
• NCBI GenBank Nucleotide #: NM_008493.3
• UniProt Accession #: P41160
• Molecular Weight: 18,709 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: Lep
• NCBI Official Synonym Symbols: ob; obese
• NCBI Protein Information: leptin; obesity factor
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: Lep
• UniProt Synonym Gene Names: Ob
• UniProt Entry Name: LEP_MOUSE

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide; Hormone

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: protein binding; growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; positive regulation of JAK-STAT cascade; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; glucose homeostasis; negative regulation of metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; regulation of metabolic process; central nervous system neuron development; placenta development; cholesterol metabolic process; positive regulation of developmental growth; regulation of protein amino acid phosphorylation; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; fatty acid catabolic process; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; positive regulation of protein import into nucleus; glycerol biosynthetic process; insulin secretion; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; lipid metabolic process; positive regulation of insulin receptor signaling pathway; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis