Leptin, Sheep Active Protein | Lep active protein

Leptin, Recombinant, Sheep (Ob, Obese Protein, Obesity Factor)

Cat. Num. AAA636533

• Gene Names: Lep; OB; obese
• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Leptin; Sheep; Recombinant; Sheep (Ob; Obese Protein; Obesity Factor); Lep active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder in 0.0045mM NaHCO3.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.

• Biological Activity: Leptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.33-0.05ng/ml.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Leptin.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC,using calibrated solution of Ovine Leptin as a Reference Standard.
• Reconstitution: Reconstitute the lyophilized Leptin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Lep active protein

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.

Recombinant Ovine Leptin is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16,130 Dalton.

Product Categories/Family for Lep active protein

Molecular Biology; MB-Obesity

NCBI and Uniprot Product Information

• NCBI GI #: 6981148
• NCBI GeneID: 25608
• NCBI Accession #: NP_037208.1
• NCBI GenBank Nucleotide #: NM_013076.3
• UniProt Accession #: P50596
• Molecular Weight: 18,866 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: Lep
• NCBI Official Synonym Symbols: OB; obese
• NCBI Protein Information: leptin; obesity factor
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: Lep
• UniProt Synonym Gene Names: Ob
• UniProt Entry Name: LEP_RAT

NCBI Description

involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]

Uniprot Description

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Subcellular location: Secreted

Probable.

Sequence similarities: Belongs to the leptin family.