leptin R4C mutant Active Protein
Ovine leptin R4C mutant
Purity
The purity of recombinant ovine leptin R4C mutantis greater than 98.0% as determined by:(a) Gel filtration analysis(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
leptin R4C mutant; Ovine leptin R4C mutant; leptin R4C mutant active protein
Host
E Coli
Purity/Purification
The purity of recombinant ovine leptin R4C mutantis greater than 98.0% as determined by:(a) Gel filtration analysis(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids of recombinant ovine leptin R4C mutant was determined and was found to be Ala-Val-Pro-Ile-Cys.
Solubility
It is recommended to reconstitute the lyophilized recombinant ovine leptin R4C mutant in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation of recombinant ovine leptin R4C mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified recombinant ovine leptin (16K) R4C mutant consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
Endotoxin content of recombinant ovine leptin is less than 0.05 ng/ug (0.5 IEU/ug) of ovine leptin R4C mutant.
Formulation
The recombinant ovine leptin R4C mutant was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
recombinant ovine leptin R4C mutant is fully biologically active compared to the wild-type ovine leptin as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Preparation and Storage
Lyophilized recombinant ovine leptin R4 degree C mutant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant ovine leptin at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant ovine leptin can be stored at 4 degree C or even room temperature for several weeks. At lower concentration of recombinant ovine leptin addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Related Product Information for leptin R4C mutant active protein
Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. The wild type leptin was mutated at position 4 replacing arginine by cysteine. Recombinant ovine leptin R4C mutant was purified by proprietary chromatographic techniques, see Reicher et al. J. Animal Sciences 90:410-418 (2012).
Product Categories/Family for leptin R4C mutant active protein
Similar Products
Product Notes
The leptin R4C mutant (Catalog #AAA400107) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of recombinan t ovine leptin R4C mutant was determined and was found to be Ala-Val-Pr o-Ile-Cys. It is sometimes possible for the material contained within the vial of "leptin R4C mutant, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
