LAG-1 Active Protein | CCL4 active protein

LAG-1 (Lymphocyte activation gene-1 protein, CCL4L1)

Cat. Num. AAA635036

• Gene Names: CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• Purity: Highly Purified; 98% by SDS-PAGE and HPLC analyses
• Synonyms: LAG-1; LAG-1 (Lymphocyte activation gene-1 protein; CCL4L1); CCL4 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE and HPLC analyses
• Form/Format: The sterile ltered solution was lyophilized with no additives.
• Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

• Biological Activity: Determined by it's ability to chemoattract CCR5 transfected cells using a concentration of 0.1-10.0ng/ml
• Country of Origin: USA
• Endotoxin Level: 0.1ng/ug (1EU/ug)
• Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other buffers and stored at 4 degree C for 1 week or stored at -20 degree C for future use.
• Preparation and Storage: -20 degree C; Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted LAG-1 should be stored in working aliquots at -20 degree C.

Related Product Information for CCL4 active protein

Lymphocyte activation gene-1 protein (LAG-1) ia a CC chemokine and is part of the MIP-1 beta family. LAG-1 is identical to MIP-beta (ACTII isotype) except for two amino acid substitutions; arginine for histidine at position 22 and serine for glycine at position 47 of the mature protein. Like MIP-1 beta, it shows in ammatory and chemokinetic properties. Lag-1 binds to both CCR5 and to CCR8. Studies have shown that LAG-1 plays an essential role in HIV suppressive factors. Recombinant Human LAG-1 is a 7.7kDa protein containing 69 amino acid residues.

Product Categories/Family for CCL4 active protein

Growth Factors; Cytokines; Growth Factors-LAG-1

NCBI and Uniprot Product Information

• NCBI GI #: 34218
• NCBI GeneID: 6351
• UniProt Accession #: P13236; P22617; Q13704; Q3SXL8; Q6FGI8
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: LAG-1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: CCL4
• NCBI Official Synonym Symbols: ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• NCBI Protein Information: C-C motif chemokine 4; PAT 744; SIS-gamma; MIP-1-beta(1-69); secreted protein G-26; T-cell activation protein 2; G-26 T-lymphocyte-secreted protein; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; small inducible cytokine A4 (homologous to mouse Mip-1b)
• UniProt Protein Name: C-C motif chemokine 4
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to toxin; response to virus; establishment and/or maintenance of cell polarity; positive regulation of calcium-mediated signaling; immune response; cell adhesion; positive regulation of calcium ion transport; signal transduction; inflammatory response; cell motility