Insulin Active Protein | INS active protein

Insulin, Recombinant, Human

Cat. Num. AAA651000

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Purity: Greater than 98.0% as determined by RP-HPLC analysis.
• Synonyms: Insulin; Recombinant; Human; INS active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by RP-HPLC analysis.
• Form/Format: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Insulin in sterile 0.005N HCl not more than 1 mg/ml.
• Biological Activity: The Biological Activity was determined to be 28 units/mg.
• Preparation and Storage: Lyophilized Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin should be stored at 4°C between 2-7 days and for future use below -18°C.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for INS active protein

Insulin Human Recombinant produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Insulin is purified by proprietary chromatographic techniques.

Product Categories/Family for INS active protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit structure: Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location: Secreted.

Involvement in disease: Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [

MIM:176730]. Ref.21 Ref.23 Ref.24 Ref.25Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [

MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Ref.34Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [

MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Ref.32 Ref.33Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [

MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Ref.33 Ref.34 Ref.35

Pharmaceutical use: Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53.

Sequence similarities: Belongs to the insulin family.

Sequence caution: The sequence AAA59179.1 differs from that shown. Reason: Erroneous gene model prediction.