Insulin-Like Growth Factor I Active Protein | IGF1 active protein
Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Applications
ELISA
Purity
Purified98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Synonyms
Insulin-Like Growth Factor I; Recombinant; Human (IGF1; IGF I); IGF1 active protein
Host
E Coli
Purity/Purification
Purified
98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Form/Format
Supplied as a lyophilized powder from 50mM acetic acid. No preservative added. Reconstitute with sterile deionized water to a concentration 100ug/ml.
Applicable Applications for IGF1 active protein
ELISA (EL/EIA)
Activity
The ED50 as determined by the dose-dependent proliferation of murine Balb/c 3T3 cells (measured by H-thymidine uptake) is <1.0ng/ml, corresponding to a specific activity of 1x10U/mg. For most in vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.
Endotoxin Level
0.1 ng/ug
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Stable for at least 6 months at -20 degree C. Reconstitute with sterile deionized water to a concentration 100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for IGF1 active protein
Recombinant Human IGF-1 is a single, non-glycosylated, polypeptide chain containing 70aa and having a molecular weight of 7,655Da. The sequence of the first five N-terminal amino acids was determined to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. Contains less than 1% dimers and aggregates.
Product Categories/Family for IGF1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~7.65kD
NCBI Official Full Name
insulin-like growth factor I
NCBI Official Synonym Full Names
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol
IGF1
NCBI Official Synonym Symbols
IGFI; IGF-I; IGF1A
NCBI Protein Information
insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB
UniProt Protein Name
Insulin-like growth factor 1 (Somatomedin C), isoform CRA_c
Protein Family
UniProt Gene Name
IGF1
UniProt Entry Name
Q14620_HUMAN
NCBI Description
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
Uniprot Description
Subcellular location: Secreted
By similarity RuleBase RU000406.
Sequence similarities: Belongs to the insulin family. RuleBase RU000406
Research Articles on IGF1
Similar Products
Product Notes
The IGF1 igf1 (Catalog #AAA650524) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Insulin-Like Growth Factor I can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Researchers should empirically determine the suitability of the IGF1 igf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin-Like Growth Factor I, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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