Insulin-Like Growth Factor Binding Protein 5 Active Protein | Igfbp5 active protein

Insulin-Like Growth Factor Binding Protein 5, Recombinant, Human (IGFBP5, IBP5, IBP-5)

Cat. Num. AAA650616

• Gene Names: Igfbp5; IGF-BP5
• Purity: Highly Purified; Chromatographically purified 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain.
• Synonyms: Insulin-Like Growth Factor Binding Protein 5; Recombinant; Human (IGFBP5; IBP5; IBP-5); Igfbp5 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; Chromatographically purified 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain.
• Form/Format: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.0. Reconstitute with sterile ddH2O to 100ug/ml
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe.

• Biological Activity: IGFBP-5 is fully biologically active when compared to standards. The ED50, calculated by its' ability to inhibit IGF-II induced proliferation of MCF-7 is <0.3µg/ml in the presence of 15ng/ml of Human IGF-II.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA,. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Igfbp5 active protein

Insulin-like growth factor binding protein 5 (IGFBP5) is one of a family of proteins that bind IGF and modulate its biological activity. IGFBP-5 is the predominant IGFBP found in bone extracts. The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and several low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. IGFBP5 is the most conserved and is developmentally up-regulated in key lineages and pathologies; in vitro studies suggest that IGFBP-5 functions independently of IGF interaction. Genetic ablation of individual IGFBP's has yielded limited phenotypes because of substantial compensation by remaining family members.

Recombinant Human IGFBP-5 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. IGFBP-5 is purified by proprietary chromatographic techniques.

Product Categories/Family for Igfbp5 active protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 6981088
• NCBI GeneID: 25285
• NCBI Accession #: NP_036949.1
• NCBI GenBank Nucleotide #: NM_012817.1
• UniProt Accession #: P24594
• Molecular Weight: 28.61kD
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGF-BP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5
• UniProt Entry Name: IBP5_RAT

NCBI Description

may be involved in ovarian folliculogenesis and may play a modulatory role in type I fiber-dominated muscles [RGD]

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Subcellular location: Secreted.

Tissue specificity: Mostly in kidney.

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.