Insulin-Like Growth Factor Binding Protein 3 Human Active Protein | IGFBP3 active protein

Insulin-Like Growth Factor Binding Protein 3, Recombinant Human (IGFBP3, tcag7.703, BP-53; IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein)

Cat. Num. AAA638095

• Gene Names: IGFBP3; IGFBP-3
• Purity: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Insulin-Like Growth Factor Binding Protein 3 Human; Insulin-Like Growth Factor Binding Protein 3; Recombinant Human (IGFBP3; tcag7.703; BP-53; IBP3; IGF-binding protein 3; Growth hormone-dependent binding protein); IGFBP3 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a sterile filtered lyophilized powder in 20mM Tris HCl. Reconstitute in sterile ddH2O, 0.1%HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly.

• Biological Activity: IGFBP-3 is fully biologically active when compared to standards. The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is less then 0.2ug/ml in the presence of 15ng/ml of Human IGF-II.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IGFBP3 active protein

Insulin Like Growth Factor Binding Protein-3 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-3 is the major IGF binding protein present in serum of humans and animals. It is also present in the alpha granules of platelets. IGFBP-3 shows a similar affinity for IGF-1 and IGF-2. IGFBP-3 inhibits follicle stimulating hormone. Markedly decreased levels of IGFBP-3 are observed in patients with growth hormone deficiencies, while markedly elevated levels are observed in patients with high levels of growth hormone (acromegaly). In murine fibroblasts the synthesis of IGFBP-3 is stimulated by mitogenic growth factors such as bombesin, vasopressin, PDGF, and EGF. In human skin fibroblasts the synthesis of IGFBP-3 is stimulated by TGF-beta.

Recombinant Human IGFBP-3 is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of 28.8kD.

Product Categories/Family for IGFBP3 active protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 27807007
• NCBI GeneID: 282261
• NCBI Accession #: NP_776981.1
• NCBI GenBank Nucleotide #: NM_174556.1
• UniProt Accession #: P20959; Q9GJV5; A6QPI3
• Molecular Weight: 28.8kD
• NCBI Official Full Name: insulin-like growth factor-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IGFBP-3
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; insulin-like growth factor binding protein-3
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_BOVIN

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Subunit structure: Interacts with HN. Interacts with TMEM219

By similarity. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with XLKD1. Ref.6

Subcellular location: Secreted.

Tissue specificity: Plasma; expressed by most tissues.

Post-translational modification: Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.