iNOS active protein

iNOS (human), (recombinant) (His-tag)

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Purity: >=90% (SDS-PAGE)
• Synonyms: iNOS; iNOS (human); (recombinant) (His-tag); iNOS active protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE)
• Form/Format: Liquid. In 50 mM TRIS, pH7.4, containing 0.1 mM EDTA, 10% glycerol, 100 mM NaCl, 0.1 mM DTT, 10uM tetrahydrobiopterin

• Source Note: Human iNOS is fused to an N-terminal His-tag
• Specific Activity: >=100U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric o)xide per minute at 37 degree C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Stable for at least 1 year after receipt when stored at -80 degree C. Keep stock vial on ice during experiments; Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for iNOS active protein

Nitric oxide synthases are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. It may function as a retrograde neurotransmitter. Nitric oxide is mediated in mammals by the calcium-calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal NOS). The inducible isoform, iNOS, is involved in immune response, binds calmodulin at physiologically relevant concentrations, and produces NO as an immune defense mechanism, as NO is a free radical with an unpaired electron. It is the proximate cause of septic shock and may function in autoimmune disease.

Product Categories/Family for iNOS active protein

Cellular Analysis; Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 131,117 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS
• UniProt Entry Name: NOS2_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; NADPH-hemoprotein reductase activity; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; receptor binding

Biological Process: circadian rhythm; interaction with host; superoxide metabolic process; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; defense response to Gram-negative bacterium; positive regulation of vasodilation; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; negative regulation of blood pressure; blood coagulation; negative regulation of protein catabolic process; inflammatory response; nitric oxide biosynthetic process; regulation of insulin secretion; nitric oxide mediated signal transduction

Disease: Hypertension, Essential; Malaria, Susceptibility To