IL3 active protein

Recombinant Mouse IL3

Cat. Num. AAA4159931

• Gene Names: Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL3; Recombinant Mouse IL3; IL-3; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; Multi-CSF; HCGF; P-cell stimulation factor; Hematopoietic growth factor; Recombinant Mouse Interleukin-3 (IL3); IL3 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS
• Sequence Length: 166

• Bioactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells was found to be in the <0.05 ng/mL
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for IL3 active protein

IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial-derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL-3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29% homology at the protein level while murine and rat IL3 show approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant mouse IL3 is a monomer of 15 kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 31982791
• NCBI GeneID: 16187
• NCBI Accession #: NP_034686.2
• NCBI GenBank Nucleotide #: NP_034686.2
• UniProt Accession #: P01586
• Molecular Weight: 15.0 kDa
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: Il3
• NCBI Official Synonym Symbols: BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• NCBI Protein Information: interleukin-3
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: Il3
• UniProt Synonym Gene Names: Csfmu; Il-3; IL-3; MCGF
• UniProt Entry Name: IL3_MOUSE

Uniprot Description

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Apoptosis; Cell cycle regulation; Cytokine; Oncoprotein; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; interleukin-3 receptor binding

Biological Process: activation of JAK protein; cytokine and chemokine mediated signaling pathway; hemopoiesis; monocyte differentiation; myeloid cell apoptosis; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; regulation of cell growth; regulation of gene expression; regulation of glycolysis; regulation of protein amino acid dephosphorylation; response to hormone stimulus; response to hypoxia