IL2 active protein

Recombinant Mouse IL2

Cat. Num. AAA4159930

• Gene Names: Il2; Il-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL2; Recombinant Mouse IL2; IL-2; T-cell growth factor; TCGF; Recombinant Mouse Interleukin-2 (IL2); IL2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from 0.2 um filtered PBS solution, pH 7.0.
• Sequence Length: 169

• Bioactivity: The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 107 units/mg.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for IL2 active protein

IL-2 is produced mainly by T cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T cell proliferation and Th1 and Th2 effector T cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 7110653
• NCBI GeneID: 16183
• NCBI Accession #: NP_032392.1
• NCBI GenBank Nucleotide #: NP_032392.1
• UniProt Accession #: P04351
• Molecular Weight: 17.0 kDa
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: Il2
• NCBI Official Synonym Symbols: Il-2
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: Il2
• UniProt Synonym Gene Names: Il-2; IL-2; TCGF
• UniProt Entry Name: IL2_MOUSE

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Cytokine

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding

Biological Process: positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; negative regulation of heart contraction; immune system process; positive regulation of immunoglobulin secretion; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of interferon-gamma production; negative regulation of protein amino acid phosphorylation; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; protein kinase C activation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of protein amino acid phosphorylation; negative regulation of B cell apoptosis