IL1RN active protein

Recombinant Human IL1RN

Cat. Num. AAA4159670

• Gene Names: IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL1RN; Recombinant Human IL1RN; IRAP; ICIL-1RA; IL1 inhibitor; IL1F3; IL-1ra; IL-1RN; Recombinant Human Interleukin-1 Receptor Antagonist (IL1RN); IL1RN active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered tris solution with sodium chloride (pH 7.5)
• Sequence Length: 143

• Bioactivity: The ED50 was determined by the ability to inhibit the IL-1 alpha mediated cell proliferation in a murine helper T cell line, for this effect, in the presence of 50 pg/mL of IL-1 alpha, is typically 20-60 ng/mL
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for IL1RN active protein

IL1RN is found in the conditioned medium of a variety of cells and is produced by macrophages, monocytes, and neutrophils. The soluble form of IL1RN has been shown to be produced by hepatocytes and to be regulated by pro-inflammatory cytokines. It may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.

NCBI and Uniprot Product Information

• NCBI GI #: 27894321
• NCBI GeneID: 3557
• NCBI Accession #: NP_776215.1
• NCBI GenBank Nucleotide #: NP_776215.1
• UniProt Accession #: P18510
• Molecular Weight: 17.0 kDa
• NCBI Official Full Name: interleukin-1 receptor antagonist protein isoform 4
• NCBI Official Synonym Full Names: interleukin 1 receptor antagonist
• NCBI Official Symbol: IL1RN
• NCBI Official Synonym Symbols: DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• NCBI Protein Information: interleukin-1 receptor antagonist protein
• UniProt Protein Name: Interleukin-1 receptor antagonist protein
• Protein Family: Interleukin-1 receptor antagonist protein
• UniProt Gene Name: IL1RN
• UniProt Synonym Gene Names: IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP
• UniProt Entry Name: IL1RA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jan 2016]

Uniprot Description

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular

Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity

Biological Process: response to drug; positive regulation of JNK activity; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; sensory perception of pain; fever; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; immune response; response to activity; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 4; Gastric Cancer, Hereditary Diffuse; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis