IGF-1 active protein

IGF-1, 49-118 aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203085

• Gene Names: IGF1; MGF; IGFI; IGF-I
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: IGF-1; 49-118 aa; Human; E Coli (Bioactivity Validated); Insulin-like growth factor-1; IGF-IA; Somatomedin-C; MGF; IBP1; IGF1; Insulin-like growth factor-1 IGF IA; IGF IB; IGFI; IGFIA; Insulin like growth factor 1; Insulin like growth factor 1 (somatomedin C); Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; Somatomedin C; ; IGF-1 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate-buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
• Sequence Length: 153

• Applicable Applications for IGF-1 active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: The ED50 for this effect is < 5ng/ml. Measured in a cell Proliferation assay using MCF7 cell.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IGF-1 active protein

The Insulin-like growth factor-1 (IGF-1) is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for IGF-1 active protein

Hormones & Cytokines

References

Rinderknecht E., et al (1978) J Biol.Chem, 253: 2769. ; Zumstein P., et al (1987) J Biol. Chem, 262: 11252.; Rabinovsky ED (2004) Neurol Res, 26: 204-210;

NCBI and Uniprot Product Information

• NCBI GI #: 11024682
• NCBI GeneID: 3479
• NCBI Accession #: NP_000609
• NCBI GenBank Nucleotide #: NM_000618.4
• UniProt Accession #: P05019; P01343; Q14620; B2RWM7; E9PD02
• Molecular Weight: 7.7kDa (71aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin like growth factor 1
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: MGF; IGFI; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IBP1; IGF-I; MGF

NCBI Description

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

Uniprot Description

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).