HIV-1 vif, Strain HXB2 Active Protein
HIV-1 vif, Strain HXB2, Recombinant (Human Immunodeficiency Virus-1)
Purity
Affinity PurifiedPurified by Nickel-affinity chromatography and the purified protein is cleaved to produce vif of 99% purity, as determined by SDS-PAGE, reduced.
Synonyms
HIV-1 vif; Strain HXB2; Recombinant (Human Immunodeficiency Virus-1); Strain HXB2 active protein
Host
E Coli
Specificity
This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Purity/Purification
Affinity Purified
Purified by Nickel-affinity chromatography and the purified protein is cleaved to produce vif of 99% purity, as determined by SDS-PAGE, reduced.
Purified by Nickel-affinity chromatography and the purified protein is cleaved to produce vif of 99% purity, as determined by SDS-PAGE, reduced.
Form/Format
Supplied as a liquid in 50mM Tris, pH 8.0, 150mM sodium chloride. No stabilizers or preservatives added.
Application Notes
Suitable for use in ELISA and Western Blot, Drug screening and Immunological studies.
Dilution: ELISA: 1-10ng.
Western Blot: 1-10ng.
Dilution: ELISA: 1-10ng.
Western Blot: 1-10ng.
Biological Activity
Vif dependent HIV-1 virus production in HIV-1 infected SupT1, Jurkat and CEM cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for HIV-1 vif, Strain HXB2 active protein
Full length Recombinant HIV-1 HXB2 vif produced as a 6xHis-fusion protein in E. coli.
Product Categories/Family for HIV-1 vif, Strain HXB2 active protein
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Product Notes
The HIV-1 vif, Strain HXB2 (Catalog #AAA637068) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in ELISA and Western Blot, Drug screening and Immunological studies. Dilution: ELISA: 1-10ng. Western Blot: 1-10ng. Researchers should empirically determine the suitability of the HIV-1 vif, Strain HXB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 vif, Strain HXB2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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