growth hormone Active Protein | gh active protein
Fish (carp) growth hormone
Purity
The purity od recombinant common carp growth hormone is greater than 95.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Synonyms
growth hormone; Fish (carp) growth hormone; Fish; common carp (Cyprinus carpio) growth hormone; gh active protein
Host
E Coli
Purity/Purification
The purity od recombinant common carp growth hormone is greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first seven N-terminal amino acids of recombinant common carp growth hormone was determined and was found to be Ser-Asp-Asn-Gln-Arg-Leu-Phe.
Sequence Length
204
Solubility
It is recommended to reconstitute the lyophilized recombinant common carp growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml. Recombinant common carp growth hormone can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content
Protein quantitation of recombinant common carp growth hormone was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.495 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.495 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard.
Dimers and Aggregates
The purified recombinant common carp growth hormone (21.4K) consists of > 98% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of recombinant common carp growth hormone.
Formulation
The recombinant common carp growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Biological Activity
Recombinant common carp growth hormone is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al.1993). Recombinant common carp growth hormone is capable to form a 1:2 complex with oGHR-ECD and induce proliferation of PFD-P1 cells stably transfected with rabbit growth hormone receptor, though its activity is 5-fold lower compared to bovine GH (A. Gertler, unpublished data).
Preparation and Storage
Lyophilized recombinant common carp growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant common carp growth hormone at > 0.1 mg/ml and filter sterilization can be stored at 4 degree C for at least two weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.
Related Product Information for gh active protein
Recombinant common carp (Cyprinus carpio) growth hormone (caGH) produced in E.Coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of 21404 Da. Recombinant common carp growth hormone is purified by chromatographic techniques, according to Fine et al. General and Comparative Endocrinology, 89,51-61 (1993).
Product Categories/Family for gh active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
23,110 Da
NCBI Official Full Name
growth hormone
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
gh
UniProt Entry Name
SOMA_ORENI
Uniprot Description
Growth hormone plays an important role in growth control and involved in the regulation of several anabolic processes.
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Product Notes
The growth hormone gh (Catalog #AAA400006) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first seven N-terminal amino acids of recombinan t common carp growth hormone was determined and was found to be Ser-Asp-As n-Gln-Arg- Leu-Phe. It is sometimes possible for the material contained within the vial of "growth hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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