Biological Activity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range )
Growth Hormone, 20kD Variant Active Protein
Growth Hormone, Recombinant, Human, 20kD Variant (hGH)
Highly Purified
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Growth Hormone; 20kD Variant; Recombinant; Human; 20kD Variant (hGH)Highly Purified; Somatotropin isoform 2; 20kDa Growth Hormone; GH; GH1; GHN; GH-N; Growth hormone; Growth hormone 1; hGH-N; Pituitary growth hormone; Somatotropin; 20kD Variant active protein
Host
E.coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Concentration
1mg/ml (determined by BCA assay) (varies by lot)
Sequence
MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF
Applicable Applications for Growth Hormone, 20kD Variant active protein
SDS-PAGE, Bioactivity
Biological Activity
Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED50 for this effect was <1ng/ml
Endotoxin Level
< 1 EU per 1ug of protein (determined by LAL method)
Domain
27-202aa
Tag
Non-tagged
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Biological Activity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range )
Biological Activity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for Growth Hormone, 20kD Variant active protein
The 20kD variant form of human growth hormone (20kD hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kD, 191 amino acids) by the deletion of amino acid residues 32-46. The physiological role of 20kD hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for Growth Hormone, 20kD Variant active protein
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Product Notes
The Growth Hormone, 20kD Variant (Catalog #AAA14756) is an Active Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Growth Hormone, 20kD Variant can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the Growth Hormone, 20kD Variant for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF. It is sometimes possible for the material contained within the vial of "Growth Hormone, 20kD Variant, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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