Growth and Differentiation Factor 5 Active Protein | Gdf5 active protein
Growth and Differentiation Factor 5, Recombinant, Human (GDF5)
Gene Names
Gdf5; Cdmp1
Purity
Highly Purified95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
Synonyms
Growth and Differentiation Factor 5; Recombinant; Human (GDF5); Gdf5 active protein
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
Form/Format
Supplied as a lyophilized powder without any additives. Reconstitute with sterile 20mM AcOH to 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA.
Biological Activity
Human GDF-5 is fully biologically active when compared to standard. Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.
Endotoxin Level
Less than 0.1ng/ug (IEU/ug) of GDF-5.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
Preparation and Storage
Lyophilized powder is stable for at least 12 months at -20 degree C Reconstituted product must be stored in aliquots at -20 degree C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Gdf5 active protein
Recombinant human GDF-5 produced in E. coli is a homodimer comprised of a non-glycosylated polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys. GDF5 is purified by proprietary chromatographic techniques.
Product Categories/Family for Gdf5 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26.8kD
NCBI Official Full Name
growth and differentiation factor-5
NCBI Official Synonym Full Names
growth differentiation factor 5
NCBI Official Symbol
Gdf5
NCBI Official Synonym Symbols
Cdmp1
NCBI Protein Information
growth differentiation factor 5; cartilage-derived morphogenetic protein 1
UniProt Protein Name
Growth and differentiation factor-5
Protein Family
UniProt Gene Name
Gdf5
UniProt Synonym Gene Names
gdf-5
UniProt Entry Name
Q5KSY1_RAT
NCBI Description
member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily; regulates cell growth and differentiation; involved with tendon and ligament formation [RGD]
Uniprot Description
Sequence similarities: Belongs to the TGF-beta family. RuleBase RU000354
Research Articles on Gdf5
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Product Notes
The Gdf5 gdf5 (Catalog #AAA650427) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Growth and Differentiation Factor 5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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