Granzyme A Active Protein | GZMA active protein

Granzyme A (human), (recombinant)

Cat. Num. AAA566052

• Gene Names: GZMA; HFSP; CTLA3
• Purity: >=98% (SDS-PAGE)
• Synonyms: Granzyme A; Granzyme A (human); (recombinant); GZMA active protein

• Host: E Coli
• Purity/Purification: >=98% (SDS-PAGE)
• Form/Format: Liquid. In 0.05M MES, pH6.0, containing 10% glycerol and ~0.52M sodium chloride Contains no preservatives
• Concentration: 0.2mg/ml (varies by lot)

• Source Note: Active human granzyme A
• Specific Activity: ~110U/ug protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25 degree C in 0.05M TRIS, pH8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Stable for at least 6 months after receipt when stored at -80 degree C. Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours; For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 degree C; Shipping: Shipped on Dry Ice

Product Categories/Family for GZMA active protein

Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• NCBI GI #: 5453676
• NCBI GeneID: 3001
• NCBI Accession #: NP_006135.1
• NCBI GenBank Nucleotide #: NM_006144.3
• UniProt Accession #: P12544; Q6IB36; A4PHN1
• Molecular Weight: 28,999 Da
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)
• UniProt Protein Name: Granzyme A
• Protein Family: Granzyme
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Apoptosis; Secreted, signal peptide; Protease; EC 3.4.21.78; Secreted

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding