Galectin-3 (LGALS3) Active Protein | LGALS3 active protein

Recombinant Human Galectin-3 (LGALS3)

Cat. Num. AAA4159796

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Galectin-3 (LGALS3); Recombinant Human Galectin-3 (LGALS3); IgE-Binding Protein; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS and 2-ME with BSA as a carrier protein
• Sequence Length: 250

• Bioactivity: Measured by its ability to agglutinate human red blood cells. The ED(50) for this effect is typically 3-10 ug/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: Reconstitute at 50 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for LGALS3 active protein

Gal-3 interacts, via its lectin domain, with glycoproteins and glycolipids on the surface of animal cells. It has been shown that nuclear Gal-3 can modulate gene expression while cytosolic Gal-3 can inhibit apoptosis and participate in exocytosis. Gal-3 is generally expressed by the epithelial cells of many tissues as well as by inflammatory cells. An increased level of Gal-3 expression may lead to some lymphomas, as well as thyroid and hepatic carcinomas while the down-regulation has been associated with breast and colon cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 115430223
• NCBI GeneID: 3958
• NCBI Accession #: NP_002297.2
• NCBI GenBank Nucleotide #: NP_002297.2
• UniProt Accession #: P17931
• Molecular Weight: 26.1 kDa
• NCBI Official Full Name: galectin-3 isoform 1
• NCBI Official Synonym Full Names: galectin 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Cell surface; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: monocyte chemotaxis; neutrophil chemotaxis; extracellular matrix organization and biogenesis; epithelial cell differentiation; positive chemotaxis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; eosinophil chemotaxis; negative regulation of T cell receptor signaling pathway; innate immune response; mRNA processing; skeletal development; macrophage chemotaxis