Fibroblast Growth Factor, Basic Active Protein
Fibroblast Growth Factor, Basic, Recombinant, Mouse (FGFb)
Purity
Highly Purified95% as determined by RP-HPLC and SDS-PAGE.
Synonyms
Fibroblast Growth Factor; Basic; Recombinant; Mouse (FGFb); Basic active protein
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by RP-HPLC and SDS-PAGE.
95% as determined by RP-HPLC and SDS-PAGE.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2, 50mM sodium sulfate, 0.2mM DTT, 0.2mM EDTA. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Ser-Gly.
Specific Activity
~2.0x10e6IU/mg
Biological Activity
The ED50, calculated by the dose-dependent proliferation of BALB/3T3 cells is <0.5ng/ml.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF-b as a Reference Standard.
1. UV spectroscopy at 280nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF-b as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Fibroblast Growth Factor, Basic active protein
FGF is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist ranging from 18-24kD in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.
Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor =FGF-7). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5).
Recombinant Mouse basic FGF (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.32kD.
Murine FGF-basic is purified by proprietary chromatographic techniques.
Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor =FGF-7). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5).
Recombinant Mouse basic FGF (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.32kD.
Murine FGF-basic is purified by proprietary chromatographic techniques.
Product Categories/Family for Fibroblast Growth Factor, Basic active protein
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Product Notes
The Fibroblast Growth Factor, Basic (Catalog #AAA650539) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Al a-Ser-Gly. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor, Basic, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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