Epiregulin Active Protein | EREG active protein

Epiregulin, Recombinant, Human (EREG)

Cat. Num. AAA650788

• Gene Names: EREG; ER
• Purity: Highly Purified; ~97% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug
• Synonyms: Epiregulin; Recombinant; Human (EREG); EREG active protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~97% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug
• Form/Format: Supplied as a sterile-filtered, lyophilized powder from 20mM PBS, pH 7.4 and 130mM sodium chloride. Reconstitute in sterile ddH2O, 01% BSA or HSA to a0.1-1mg/ml.

• Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is <2ng/ml, corresponding to a specific activity of 5x10e5units/mg.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for EREG active protein

Epiregulin is an EGF related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes and in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kD mature secreted sequence. Recombinant human Epiregulin is a 6.0kD monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.

Product Categories/Family for EREG active protein

Growth Factors; Cytokines; Growth Factors-Epiregulin

NCBI and Uniprot Product Information

• NCBI GI #: 2381481
• NCBI GeneID: 2069
• UniProt Accession #: O14944; Q6FH69
• Molecular Weight: 6kD
• NCBI Official Full Name: epiregulin
• NCBI Official Synonym Full Names: epiregulin
• NCBI Official Symbol: EREG
• NCBI Official Synonym Symbols: ER
• NCBI Protein Information: proepiregulin; OTTHUMP00000160472
• UniProt Protein Name: Proepiregulin
• Protein Family: Proepiregulin
• UniProt Gene Name: EREG
• UniProt Entry Name: EREG_HUMAN

NCBI Description

Epiregulin is a member of the epidermal growth factor family. Epiregulin can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. [provided by RefSeq]

Uniprot Description

Function: May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.

Subcellular location: Epiregulin: Secreted › extracellular space. Proepiregulin: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon.

Sequence similarities: Contains 1 EGF-like domain.