Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a Active Protein | TPK1 active protein
Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a, Recombinant (cAMP PKA R1)
Gene Names
TPK1; PKA1; SRA3
Purity
Highly Purified90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a; Recombinant (cAMP PKA R1); TPK1 active protein
Host
E Coli
Purity/Purification
Highly Purified
90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a liquid in 50% glycerol.
Biological Activity
Recombinant Human PKA regulatory subunit alpha specifically inhibits PKA catalytic subunit (Ki about 0.1nM). Activity can be restored by adding cAMP (Kact about 100nM). The binding of the catalytic subunit is dependent on the presence of ATP and Mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Unit Definition
One unit is defined as the amount of recombinant PKA catalytic subunit alpha, required to incorporate 1nmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30 degree C.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for TPK1 active protein
cAMP-dependent PKA is an ubiquitous serine/theonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catatytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA regulatory subunit I a is a dimeric 90kD protein.
Product Categories/Family for TPK1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,076 Da
NCBI Official Full Name
cAMP-dependent protein kinase type 1
NCBI Official Symbol
TPK1
NCBI Official Synonym Symbols
PKA1; SRA3
NCBI Protein Information
Tpk1p
UniProt Protein Name
cAMP-dependent protein kinase type 1
Protein Family
UniProt Gene Name
TPK1
UniProt Synonym Gene Names
PKA1; SRA3; PKA 1
UniProt Entry Name
KAPA_YEAST
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Activated by cAMP.
Subcellular location: Cytoplasm. Nucleus Ref.7.
Miscellaneous: Present with 4320 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.
Sequence caution: The sequence AAA35088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Research Articles on TPK1
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Product Notes
The TPK1 tpk1 (Catalog #AAA635707) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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