Activin-A Active Protein | INHBA active protein

Recombinant Human Activin-A, Active

Cat. Num. AAA145200

• Gene Names: INHBA; EDF; FRP
• Purity: Greater than 95% as obsereved by SDS-PAGE.
• Synonyms: Activin-A; Recombinant Human Activin-A; Active; Inhba; Inhibin beta A; FSH releasing protein.; INHBA active protein

• Host: E Coli
• Purity/Purification: Greater than 95% as obsereved by SDS-PAGE.
• Form/Format: Human Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 0.1% TFA.; Lyophilized freeze dried powder.
• Sequence: MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS
• Sequence Length: 426

• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Human INHBA protein should be reconstituted in distilled pyrogen free water to a concentration of 100ug /ml which can then be further diluted to other aqueous solutions.
• Biological Activity: Biological activity is assessed by the ability to induce cytoxicity of MPC-11 cells and was found to be 8.8ng/ml corresponding to a specific activity of 1.1x10⁵ units/mg.
• Preparation and Storage: Lyophilized Activin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Activin-A should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for INHBA active protein

Description: Active form Activin-A Human Recombinant produced in E Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2kDa.The Active form Activin-A is purified by standard chromatographic techniques.

Introduction: Activins are homodimers or heterodimers of the different beta subunit isoforms, part of the TGFbeta family. Mature Activin A has two 116 amino acids residues betaA subunits (betaA-betaA). Activin displays an extensive variety of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins takes part in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells that are identified to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.

Product Categories/Family for INHBA active protein

Cytokines and Growth Factors; Growth Factors; Activin

NCBI and Uniprot Product Information

• NCBI GI #: 4504699
• NCBI GeneID: 3624
• NCBI Accession #: NP_002183.1
• NCBI GenBank Nucleotide #: NM_002192.2
• UniProt Accession #: P08476; Q14599
• NCBI Official Full Name: inhibin beta A chain
• NCBI Official Synonym Full Names: inhibin, beta A
• NCBI Official Symbol: INHBA
• NCBI Official Synonym Symbols: EDF; FRP
• NCBI Protein Information: inhibin beta A chain; FSH-releasing protein; Inhibin, beta-1; activin beta-A chain; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)
• UniProt Protein Name: Inhibin beta A chain
• Protein Family: Inhibin
• UniProt Gene Name: INHBA
• UniProt Synonym Gene Names: EDF
• UniProt Entry Name: INHBA_HUMAN

NCBI Description

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; cell-cell signaling; ovarian follicle development; hair follicle development; erythrocyte differentiation; cell cycle arrest; cell differentiation; response to drug; hemoglobin biosynthetic process; negative regulation of B cell differentiation; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; G1/S transition of mitotic cell cycle; growth