Dual Specificity Phosphatase 6 Recombinant Protein | DUSP6 recombinant protein

Recombinant Dual Specificity Phosphatase 6 (DUSP6)

Cat. Num. AAA2029104

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Dual Specificity Phosphatase 6; Recombinant Dual Specificity Phosphatase 6 (DUSP6); DUSP6 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MIDTLRPVPF ASEMAISKTV AWLNEQLELG NERLLLMDCR PQELYESSHIESAINVAIPG IMLRRLQKGN LPVRALFTRG EDRDRFTRRC GTDTVVLYDESSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFSLHCETNLDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLSNSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENAGEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS
• Sequence Length: 381

• Applicable Applications for DUSP6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.9kDa
• Accurate Molecular Mass (KD): 37kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Ser300
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 30582629
• NCBI GeneID: 1848
• Molecular Weight: 26,468 Da
• NCBI Official Full Name: dual specificity phosphatase 6
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6
• UniProt Protein Name: Dual specificity protein phosphatase 6
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP6
• UniProt Synonym Gene Names: MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
• UniProt Entry Name: DUS6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: response to drug; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of fibroblast growth factor receptor signaling pathway; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; response to nitrosative stress; dorsal/ventral pattern formation; toll-like receptor signaling pathway; innate immune response; regulation of endodermal cell fate specification; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway; inactivation of MAPK activity

Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia