SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
DTYMK recombinant protein
DTYMK, 1-212aa, Human, His tag, E Coli
Gene Names
DTYMK; CDC8; TMPK; TYMK; PP3731
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
DTYMK; 1-212aa; Human; His tag; E Coli; Thymidylate kinase; CDC8; FLJ44192; MGC198617; PP3731; TMPK; TYMK; DTYMK recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK
Sequence Length
212
Applicable Applications for DTYMK recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
1-212aa
Preparation and Storage
Can be stored at 2°C to 8°C for 1week. For long term storage , aliqout and store at -20°C to -80°C
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for DTYMK recombinant protein
DTYMK is also known as thymidylate kinase family and is involved in pyrimidine metabolism. Specifically, DTYMK catalyzes the ATP-dependent conversion of dTMP (deoxythymidine monophosphate) to dTDP (deoxythymidine diphosphate), which then functions as one of the four nucleotides in DNA. Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth. Recombinant human DTYMK protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DTYMK recombinant protein
References
Cummins RR., et al. (1980) Cancer Res. 40 (4):1235-9.; Su JY., et al. (1991) Nucleic Acids Res. 19 (4):823-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26 kDa (232aa), confirmed by MALDI-TOF
NCBI Official Full Name
thymidylate kinase isoform 1
NCBI Official Synonym Full Names
deoxythymidylate kinase
NCBI Official Symbol
DTYMK
NCBI Official Synonym Symbols
CDC8; TMPK; TYMK; PP3731
NCBI Protein Information
thymidylate kinase
UniProt Protein Name
Thymidylate kinase
UniProt Gene Name
DTYMK
UniProt Synonym Gene Names
CDC8; TMPK; TYMK
Uniprot Description
Catalyzes the conversion of dTMP to dTDP.
Research Articles on DTYMK
Similar Products
Product Notes
The DTYMK dtymk (Catalog #AAA204368) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DTYMK can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DTYMK dtymk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK. It is sometimes possible for the material contained within the vial of "DTYMK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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