Mouse Dentin Sialophosphoprotein ELISA Kit | DSPP elisa kit

Mouse Dentin Sialophosphoprotein ELISA Kit

Cat. Num. AAA720883

• Gene Names: DSPP; DPP; DSP; DGI1; DMP3; DTDP2; DFNA39
• Reactivity: Mouse
• Synonyms: Dentin Sialophosphoprotein; Mouse Dentin Sialophosphoprotein ELISA Kit; DSPP elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Intended Uses: This DSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DSP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This DSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DSP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DSPP elisa kit

Principle of the Assay: DSP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DSP. Standards or samples are then added to the microtiter plate wells and DSP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DSP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DSP are added to each well to "sandwich" the DSP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DSP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DSPP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 89001107
• NCBI GeneID: 1834
• NCBI Accession #: NP_055023.2
• NCBI GenBank Nucleotide #: NM_014208.3
• Molecular Weight: 131,151 Da
• NCBI Official Full Name: dentin sialophosphoprotein preproprotein
• NCBI Official Synonym Full Names: dentin sialophosphoprotein
• NCBI Official Symbol: DSPP
• NCBI Official Synonym Symbols: DPP; DSP; DGI1; DMP3; DTDP2; DFNA39
• NCBI Protein Information: dentin sialophosphoprotein; dentin phosphoryn; dentin sialoprotein; dentin phosphophoryn; dentin phosphoprotein
• UniProt Protein Name: Dentin sialophosphoprotein
• Protein Family: Dentin sialophosphoprotein
• UniProt Gene Name: DSPP
• UniProt Synonym Gene Names: DPP; DSP
• UniProt Entry Name: DSPP_HUMAN

NCBI Description

This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DSPP: DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals. Defects in DSPP are the cause of deafness autosomal dominant type 39 with dentinogenesis imperfecta 1 (DFNA39/DGI1). Affected individuals present DGI1 associated with early onset progressive sensorineural high-frequency hearing loss. Defects in DSPP are the cause of dentinogenesis imperfecta type 1 (DGI1); also known as dentinogenesis imperfecta Shields type 2 (DGI2). DGI1 is an autosomal dominant disorder in which both the primary and the permanent teeth are affected. It occurs with an incidence of 1:8000 live births. The teeth are amber and opalescent, the pulp chamber being obliterated by abnormal dentin. The enamel, although unaffected, tends to fracture, which makes dentin undergo rapid attrition, leading to shortening of the teeth. Defects in DSPP are a cause of dentinogenesis imperfecta Shields type 3 (DGI3). Patients with DGI3 do not have stigmata of osteogenesis imperfecta. The finding that a single defects in the DSPP gene causes both phenotypic patterns of DGI2 and DGI3 strongly supports the conclusion that these two disorders are not separate diseases but rather the phenotypic variation of a single genetic defect. Defects in DSPP are the cause of dentin dysplasia type 2 (DTDP2); also known as dentin dysplasia Shields type 2. DTDP2 is an autosomal dominant disorder in which mineralization of the dentine of the primary teeth is abnormal. On the basis of the phenotypic overlap between, and shared chromosomal location with DGI2 it has been proposed that DTDP2 and DGI2 are allelic. From the results of recent studies, it is clear that different types of mutations in DSPP lead to the two different phenotypes.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: collagen binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; ossification; biomineral formation; multicellular organismal development; skeletal development

Disease: Dentinogenesis Imperfecta, Shields Type Iii; Deafness, Autosomal Dominant 39, With Dentinogenesis Imperfecta 1; Dentin Dysplasia, Type Ii; Dentinogenesis Imperfecta 1