Desmocollin 2/DSC2 Recombinant Protein | DSC2 recombinant protein

Desmocollin 2/DSC2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120497

• Gene Names: DSC2; DG2; DSC3; CDHF2; ARVD11; DGII/III
• Purity: >75% as determined by SDS-PAGE
• Synonyms: Desmocollin 2/DSC2; Desmocollin 2/DSC2 Protein; Human; Recombinant (His Tag); Human DSC2/Desmocollin-2 Protein (His Tag); ARVD11 Protein; CDHF2 Protein; DG2 Protein; DGII/III Protein; DSC3 Protein; desmocollin 2; DSC2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >75% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg684

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 28 & Arg 136
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Arg 684) of human DSC2 (Q02487-1) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DSC2 recombinant protein

Background: DSC2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in DSC2 are associated with arrhythmogenic right ventricular dysplasia-11. DSC2 is Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSC2 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms.

References

Nuber UA, et al. (1995) The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes. Eur J Cell Biol. 66 (1): 69-74.Marsden MD, et al. (1997) Cloning and transcriptional analysis of the promoter of the human type 2 desmocollin gene (DSC2). Gene. 186 (2): 237-47.Greenwood MD, et al. (1997) Exon-intron organization of the human type 2 desmocollin gene (DSC2): desmocollin gene structure is closer to "classical" cadherins than to desmogleins. Genomics. 44 (3): 330-5.

NCBI and Uniprot Product Information

• NCBI GI #: 38648769
• NCBI GeneID: 1824
• UniProt Accession #: Q02487
• Molecular Weight: 93,769 Da
• NCBI Official Full Name: Desmocollin 2
• NCBI Official Synonym Full Names: desmocollin 2
• NCBI Official Symbol: DSC2
• NCBI Official Synonym Symbols: DG2; DSC3; CDHF2; ARVD11; DGII/III
• NCBI Protein Information: desmocollin-2
• UniProt Protein Name: Desmocollin-2
• Protein Family: Desmocollin
• UniProt Gene Name: DSC2

NCBI Description

This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.