Pig D (1A) dopamine receptor ELISA Kit | DRD1 elisa kit
Pig D (1A) dopamine receptor (DRD1) ELISA Kit
Reactivity
Pig
Synonyms
D (1A) dopamine receptor; Pig D (1A) dopamine receptor (DRD1) ELISA Kit; DRD1 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig DRD1. No significant cross-reactivity or interference between Pig DRD1 and analogues was observed.
Sequence Length
446
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DRD1 elisa kit
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
DRD1 gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DRD1 in samples. An antibody specific for DRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDRD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
DRD1 gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DRD1 in samples. An antibody specific for DRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDRD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,257 Da
NCBI Official Full Name
D(1A) dopamine receptor
NCBI Official Symbol
DRD1
NCBI Protein Information
D(1A) dopamine receptor
UniProt Protein Name
D(1A) dopamine receptor
Protein Family
UniProt Gene Name
DRD1
Uniprot Description
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Research Articles on DRD1
Similar Products
Product Notes
The Pig DRD1 drd1 (Catalog #AAA280743) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280743 ELISA Kit recognizes Pig DRD1. It is sometimes possible for the material contained within the vial of "D (1A) dopamine receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
