Developmental Pluripotency Associated Protein 3 Recombinant Protein | DPPA3 recombinant protein

Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3)

Cat. Num. AAA2033108

• Gene Names: DPPA3; STELLA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Developmental Pluripotency Associated Protein 3; Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3); STELLA; STELLAR; Stella-related protein; DPPA3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MDPSQFNPTY IPGSPQMLTE ENSRDDSGAS QISSETLIKN LSNLTINASS ESVSPLSEAL LRRESVGAAV LREIEDEWLY SRRGVRTLLS VQREKMARLR YMLLGGVRTH ERRPTNKEPKGVKKESRPFK CPCSFCVSNG WDPSENARIG NQDTKPLQP
• Sequence Length: 159

• Applicable Applications for DPPA3 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 21.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Pro159
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40548326
• NCBI GeneID: 359787
• NCBI Accession #: NP_954980.1
• NCBI GenBank Nucleotide #: NM_199286.3
• Molecular Weight: 17,851 Da
• NCBI Official Full Name: developmental pluripotency-associated protein 3
• NCBI Official Synonym Full Names: developmental pluripotency associated 3
• NCBI Official Symbol: DPPA3
• NCBI Official Synonym Symbols: STELLA
• NCBI Protein Information: developmental pluripotency-associated protein 3
• UniProt Protein Name: Developmental pluripotency-associated protein 3
• Protein Family: Developmental pluripotency-associated protein
• UniProt Gene Name: DPPA3
• UniProt Synonym Gene Names: STELLAR
• UniProt Entry Name: DPPA3_HUMAN

NCBI Description

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008]

Uniprot Description

DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytoplasm; female pronucleus; male pronucleus; nucleus

Molecular Function: methylated histone residue binding

Biological Process: chromatin modification; embryonic cleavage