Dipeptidyl Peptidase 4 (Dpp4) Recombinant Protein | Dpp4 recombinant protein

Recombinant Rat Dipeptidyl Peptidase 4 (Dpp4), Partial

Cat. Num. AAA7115351

• Gene Names: Dpp4; CD26; DPPIV
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Dipeptidyl Peptidase 4 (Dpp4); Recombinant Rat Dipeptidyl Peptidase 4 (Dpp4); Partial; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; Dpp4 recombinant protein

• Host: In Vitro E Coli Expression System
• Specificity: Expressed in bile ducts and other epithelial brush borders (small intestine, kidney, colon, pancreatic duct); acinar structures in salivary glands; endothelial structures and T cell areas in thymus, spleen and lymph node.
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Tris-Based Buffer, 50% Glycerol
• Sequence Positions: 37-767. Partial,provide Dipeptidyl peptidase 4 soluble form chain
• Sequence: SRRTYTLADYLKNTFRVKSYSLRWVSDSEYLYKQENNILLFNAEHGNSSIFLENSTFEIFGDSISDYSVSPDRLFVLLEYNYVKQWRHSYTASYSIYDLNKRQLITEEKIPNNTQWITWSQEGHKLAYVWKNDIYVKIEPHLPSHRITSTGKENVIFNGINDWVYEEEIFGAYSALWWSPNGTFLAYAQFNDTGVPLIEYSFYSDESLQYPKTVWIPYPKAGAVNPTVKFFIVNTDSLSSTTTTIPMQITAPASVTTGDHYLCDVAWVSEDRISLQWLRRIQNYSVMAICDYDKTTLVWNCPTTQEHIETSATGWCGRFRPAEPHFTSDGSSFYKIVSDKDGYKHICQFQKDRKPEQVCTFITKGAWEVISIEALTSDYLYYISNEYKEMPGGRNLYKIQLTDHTNKKCLSCDLNPERCQYYSVSLSKEAKYYQLGCRGPGLPLYTLHRSTDQKELRVLEDNSALDKMLQDVQMPSKKLDFIVLNETRFWYQMILPPHFDKSKKYPLLIDVYAGPCSQKADAAFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINKRLGTLEVEDQIEAARQFLKMGFVDSKRVAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYSHMSHFLQQCFSLR

• Species: Rat
• Tag: N-terminal 6xHis-tagged
• Subcellular Location: Dipeptidyl peptidase 4 soluble form: Secreted, Note=Detected in the serum and the seminal fluid, SUBCELLULAR LOCATION: Cell membrane, Single-pass type II membrane protein, Apical cell membrane, Single-pass type II membrane protein, Cell projection, invadopodium membrane, Single-pass type II membrane protein, Cell projection, lamellipodium membrane, Single-pass type II membrane protein, Cell junction, Membrane raft
• Protein Families: Peptidase S9B family, DPPIV subfamily
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for Dpp4 recombinant protein

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

Product Categories/Family for Dpp4 recombinant protein

Cancer

References

"Primary structure of rat liver dipeptidyl peptidase IV deduced from its cDNA and identification of the NH2-terminal signal sequence as the membrane-anchoring domain." Ogata S., Misumi Y., Ikehara Y. J. Biol. Chem. 264:3596-3601(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 6978773
• NCBI GeneID: 25253
• NCBI Accession #: NP_036921.1
• NCBI GenBank Nucleotide #: NM_012789.1
• UniProt Accession #: P14740
• Molecular Weight: 88.3 kDa
• NCBI Official Full Name: dipeptidyl peptidase 4
• NCBI Official Synonym Full Names: dipeptidylpeptidase 4
• NCBI Official Symbol: Dpp4
• NCBI Official Synonym Symbols: CD26; DPPIV
• NCBI Protein Information: dipeptidyl peptidase 4
• UniProt Protein Name: Dipeptidyl peptidase 4
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: Dpp4
• UniProt Synonym Gene Names: Cd26; DPP IV
• UniProt Entry Name: DPP4_RAT

NCBI Description

catalyzes the degradation of glucagon-like peptide-1 (Glp-1); involved in proteolysis and peptidolysis [RGD, Feb 2006]

Uniprot Description

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Endoplasmic reticulum; Vesicle; Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion; Protease; Cell surface; Cell cycle regulation; EC 3.4.14.5

Cellular Component: Golgi apparatus; intercellular canaliculus; cell surface; focal adhesion; lysosomal membrane; endoplasmic reticulum; integral to membrane; extracellular region; lipid raft; membrane; lamellipodium; endocytic vesicle; apical plasma membrane; plasma membrane; cell junction

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; protease binding; serine-type peptidase activity; dipeptidyl-peptidase activity; serine-type endopeptidase activity; peptide binding; receptor binding

Biological Process: regulation of cell-cell adhesion mediated by integrin; behavioral fear response; T cell activation; establishment of localization; regulation of T cell mediated immunity; positive regulation of cell proliferation; T cell costimulation; response to hypoxia; endothelial cell migration; cell adhesion; proteolysis