DPP4/CD26 Recombinant Protein | DPP4 recombinant protein

DPP4/CD26 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122945

• Gene Names: Dpp4; Cd26; THAM; Dpp-4
• Purity: >90% as determined by SDS-PAGE
• Synonyms: DPP4/CD26; DPP4/CD26 Protein; Mouse; Recombinant (His Tag); Mouse DPP4/CD26 Protein (His Tag); Cd26 Protein; Dpp-4 Protein; THAM Protein; dipeptidylpeptidase 4; DPP4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser29-His760

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Dpp4 (NP_034204.1)(Ser29-His760) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for DPP4 recombinant protein

Background: Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Product Categories/Family for DPP4 recombinant protein

Serine Proteases and Regulators

References

Doupis J, et al. (2008) DPP4 inhibitors: a new approach in diabetes treatment. Adv Ther. 25(7): 627-43.Havre PA, et al. (2008) The role of CD26/dipeptidyl peptidase IV in cancer. Front Biosci. 13: 1634-45.De Chiara L, et al. (2009) Soluble CD26 levels and its association to epidemiologic parameters in a sample population. Dis Markers. 7(6): 311-6.Matteucci E, et al. (2009) Dipeptidyl peptidase-4 (CD26): knowing the function before inhibiting the enzyme. Curr Med Chem. 16(23): 2943-51.

NCBI and Uniprot Product Information

• NCBI GI #: 227116292
• NCBI GeneID: 13482
• NCBI Accession #: NP_034204.1
• NCBI GenBank Nucleotide #: NM_001159543.1
• UniProt Accession #: P28843; Q3U514
• Molecular Weight: 87,437 Da
• NCBI Official Full Name: dipeptidyl peptidase 4 isoform 2
• NCBI Official Synonym Full Names: dipeptidylpeptidase 4
• NCBI Official Symbol: Dpp4
• NCBI Official Synonym Symbols: Cd26; THAM; Dpp-4
• NCBI Protein Information: dipeptidyl peptidase 4; DPP IV; dipeptidyl peptidase IV; thymocyte-activating molecule; T-cell activation antigen CD26
• UniProt Protein Name: Dipeptidyl peptidase 4
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: Dpp4
• UniProt Synonym Gene Names: Cd26; DPP IV; THAM
• UniProt Entry Name: DPP4_MOUSE

Uniprot Description

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Membrane protein, integral; Cell adhesion; EC 3.4.14.5; Cell surface; Protease

Cellular Component: Golgi apparatus; intercellular canaliculus; focal adhesion; cell surface; endoplasmic reticulum; lysosomal membrane; extracellular region; integral to membrane; cell projection; membrane; endocytic vesicle; lamellipodium; apical plasma membrane; plasma membrane; cell junction

Molecular Function: peptidase activity; collagen binding; identical protein binding; protein homodimerization activity; protease binding; hydrolase activity; serine-type peptidase activity; dipeptidyl-peptidase activity; serine-type endopeptidase activity; aminopeptidase activity; peptide binding; receptor binding

Biological Process: regulation of cell-cell adhesion mediated by integrin; behavioral fear response; T cell activation; establishment of localization; regulation of T cell mediated immunity; positive regulation of cell proliferation; T cell costimulation; response to hypoxia; endothelial cell migration; proteolysis; cell adhesion