DPH2 recombinant protein

DPH2, 1-489aa, Human, His-tag, Baculovirus

Cat. Num. AAA206632

• Gene Names: DPH2; DPH2L2
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: DPH2; 1-489aa; Human; His-tag; Baculovirus; Diphthamide biosynthesis protein 2; DPH2L2; DPH2 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 1mM DTT, 20% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH
• Sequence Length: 489

• Applicable Applications for DPH2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DPH2 recombinant protein

DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for DPH2 recombinant protein

Cell Cycle; Other

References

Zhang Y., et al. (2010) Nature. 465:891-896.; Mattheakis LC., et al. (1993) Gene. 132:149-154.

NCBI and Uniprot Product Information

• NCBI GI #: 21536476
• NCBI GeneID: 1802
• NCBI Accession #: NP_001375
• NCBI GenBank Nucleotide #: NM_001384.4
• UniProt Accession #: Q9BQC3; O60623; A8MVC9; B2RDE3; B4DNI8
• Molecular Weight: 53.1kDa (497aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform a
• NCBI Official Synonym Full Names: DPH2 homolog
• NCBI Official Symbol: DPH2
• NCBI Official Synonym Symbols: DPH2L2
• NCBI Protein Information: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2; diphthamide biosynthesis protein 2
• UniProt Protein Name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
• Protein Family: 2-(3-amino-3-carboxypropyl)histidine synthase
• UniProt Gene Name: DPH2
• UniProt Synonym Gene Names: DPH2L2

NCBI Description

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).