SDS-Page
Docking Protein 1 Recombinant Protein | DOK1 recombinant protein
Recombinant Docking Protein 1 (DOK1)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Docking Protein 1; Recombinant Docking Protein 1 (DOK1); P62DOK; Downstream Of Tyrosine Kinase 1; DOK1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
GTK RWKKTWAVLY PASPHGVARL EFFDHKGSSS GGGRGGSRRL DCKMIRLAEC VSVVPVTVES PPEPGASAFR LDTAQRSHLL AADAASSTAW VQILCRTAFP KGGWALAQTE NPPKFSALEM LENSLYSPTW EGSQFWVTSQ KTEASERCGL QGSYVLRVEA EKLTLLTLGAQSQILEPLLF WPYTLLRRYG RDKVMFSFEA GRRCPSGPGT FTFQTAQ
Sequence Length
483
Applicable Applications for DOK1 recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
28.0kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Gly18~Gln237
Organism Species
Rat
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,206 Da
NCBI Official Full Name
docking protein 1
NCBI Official Symbol
DOK1
NCBI Protein Information
docking protein 1
UniProt Protein Name
Docking protein 1
Protein Family
UniProt Gene Name
DOK1
UniProt Entry Name
DOK1_BOVIN
Uniprot Description
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 ().
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Product Notes
The DOK1 dok1 (Catalog #AAA2033562) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Docking Protein 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the DOK1 dok1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GTK RWKKTWAVLY PASPHGVARL EFFDHKGSSS GGGRGGSRRL DCKMIRLAEC VSVVPVTVES PPEPGASAFR LDTAQRSHLL AADAASSTAW VQILCRTAFP KGGWALAQTE NPPKFSALEM LENSLYSPTW EGSQFWVTSQ KTEASERCGL QGSYVLRVEA EKLTLLTLGA QSQILEPLLF WPYTLLRRYG RDKVMFSFEA GRRCPSGPGT FTFQTAQ. It is sometimes possible for the material contained within the vial of "Docking Protein 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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