Dog Vascular Endothelial Growth Factor A (VEGFA) RTU ELISA Kit | VEGFA rtu elisa kit

Canine Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit

Cat. Num. AAA4503288

• Gene Names: VEGFA; VEGF
• Reactivity: Dog
• Synonyms: Vascular Endothelial Growth Factor A (VEGFA); Canine Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit; VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor; VEGFA rtu elisa kit

• Reactivity: Dog
• Sequence Length: 214

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFA rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160358860
• NCBI GeneID: 403802
• NCBI Accession #: NP_001003175.2
• NCBI GenBank Nucleotide #: NM_001003175.2
• UniProt Accession #: Q9MYV3; Q9XSF3; Q9XSF4; Q9XSF5
• Molecular Weight: 22,292 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform 1
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VEGF
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().