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Dog Albumin ELISA Kit | ALB elisa kit

Dog Albumin ELISA Kit

Gene Names
ALB; PRO0883; PRO0903; PRO1341; DKFZp779N1935
Reactivity
Dog
Synonyms
Albumin; Dog Albumin ELISA Kit; Dog Albumin; ALB elisa kit
Ordering
For Research Use Only!
Reactivity
Dog
Samples
Immunoperoxidase Assay for Determination of Albumin in Dog Samples
Assay Type
Sandwich
Intended Uses
The Albumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Dogs.
Preparation and Storage
Store at 4 degree C.
Related Product Information for ALB elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Albumin is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.
Product Categories/Family for ALB elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
213
Molecular Weight
69,367 Da
NCBI Official Full Name
albumin
NCBI Official Synonym Full Names
albumin
NCBI Official Symbol
ALB
NCBI Official Synonym Symbols
PRO0883; PRO0903; PRO1341; DKFZp779N1935
NCBI Protein Information
serum albumin; OTTHUMP00000160370; OTTHUMP00000196832; OTTHUMP00000220435; OTTHUMP00000220436; OTTHUMP00000220438; OTTHUMP00000220439; growth-inhibiting protein 20; cell growth inhibiting protein 42
UniProt Protein Name
Serum albumin
Protein Family
UniProt Gene Name
ALB
UniProt Entry Name
ALBU_HUMAN

NCBI Description

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carrier; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; enzyme binding; copper ion binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; maintenance of mitochondrion localization; bile acid metabolic process; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; positive regulation of circadian sleep/wake cycle, non-REM sleep; transmembrane transport; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

Research Articles on ALB

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Product Notes

The Dog ALB alb (Catalog #AAA564011) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564011 ELISA Kit recognizes Dog ALB. It is sometimes possible for the material contained within the vial of "Albumin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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