Pig Dihydrolipoyl dehydrogenase ELISA Kit | DLD elisa kit

Pig Dihydrolipoyl dehydrogenase, mitochondrial (DLD) ELISA Kit

Cat. Num. AAA282071

• Gene Names: DLD; DLDH
• Reactivity: Pig
• Synonyms: Dihydrolipoyl dehydrogenase; Pig Dihydrolipoyl dehydrogenase; mitochondrial (DLD) ELISA Kit; DLD elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig DLD. No significant cross-reactivity or interference between Pig DLD and analogues was observed.
• Sequence Length: 477

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DLD elisa kit

The dihydrolipoate, still bound to a lysine residue of the complex, then migrates to the dihydrolipoyl dehydrogenase (E3) active site where it undergoes a flavin-mediated oxidation, identical in chemistry to disulfide isomerase. First, FAD oxidizes dihydrolipoate back to its lipoate resting state, producing FADH2.
Dihydrolipoamide dehydrogenase is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DLD in samples. An antibody specific for DLD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLD bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 47522940
• NCBI GeneID: 397129
• NCBI Accession #: NP_999227.1
• NCBI GenBank Nucleotide #: NM_214062.1
• UniProt Accession #: P09623
• Molecular Weight: 54,185 Da
• NCBI Official Full Name: dihydrolipoyl dehydrogenase, mitochondrial
• NCBI Official Symbol: DLD
• NCBI Official Synonym Symbols: DLDH
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• UniProt Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein
• UniProt Gene Name: DLD
• UniProt Synonym Gene Names: LAD

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]

Uniprot Description

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of the alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form has additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction ().