DKK3 recombinant protein

DKK3, 22-350aa, Human, His-tag, Baculovirus

Cat. Num. AAA206526

• Gene Names: DKK3; RIG; REIC
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DKK3; 22-350aa; Human; His-tag; Baculovirus; dickkopf WNT signaling pathway inhibitor 3; RIG; REIC; DKK3 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPAPAPTAT SAPVKPGPAL SYPQEEATLN EMFREVEELM EDTQHKLRSA VEEMEAEEAA AKASSEVNLA NLPPSYHNET NTDTKVGNNT IHVHREIHKI TNNQTGQMVF SETVITSVGD EEGRRSHECI IDEDCGPSMY CQFASFQYTC QPCRGQRMLC TRDSECCGDQ LCVWGHCTKM ATRGSNGTIC DNQRDCQPGL CCAFQRGLLF PVCTPLPVEG ELCHDPASRL LDLITWELEP DGALDRCPCA SGLLCQPHSH SLVYVCKPTF VGSRDQDGEI LLPREVPDEY EVGSFMEEVR QELEDLERSL TEEMALREPA AAAAALLGGE EIHHHHHH
• Sequence Length: 350

• Applicable Applications for DKK3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DKK3 recombinant protein

DKK3, also known as dickkopf WNT signaling pathway inhibitor 3, is a member of the DKK protein family including Dkk-1, 2, 3 and 4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Recombinant human DKK3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for DKK3 recombinant protein

Signal Transduction

References

Kim BR., et al. (2015) Cell Signal. 27: 2150-2159; Sakaguchi M., et al. (2011) Adv Exp Med Biol. 720: 209-215.

NCBI and Uniprot Product Information

• NCBI GI #: 66346689
• NCBI GeneID: 27122
• NCBI Accession #: NP_001018067
• NCBI GenBank Nucleotide #: NM_001018057.1
• UniProt Accession #: Q9UBP4; Q9ULB7; A8K1I2; D3DQW1
• Molecular Weight: 37.3kDa (338aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: dickkopf-related protein 3 isoform 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 3
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3
• UniProt Protein Name: Dickkopf-related protein 3
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Synonym Gene Names: REIC; Dickkopf-3; Dkk-3; hDkk-3

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease ().