Mouse Deoxyhypusine synthase ELISA Kit | DHPS elisa kit

Mouse Deoxyhypusine synthase (DHPS) ELISA Kit

Cat. Num. AAA285882

• Gene Names: Dhps; Dhs
• Reactivity: Mouse
• Synonyms: Deoxyhypusine synthase; Mouse Deoxyhypusine synthase (DHPS) ELISA Kit; MIG13; migration-inducing gene 13; DHPS elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse DHPS. No significant cross-reactivity or interference between Mouse DHPS and analogues was observed.
• Sequence Length: 369

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DHPS elisa kit

The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A.In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHPS in samples. An antibody specific for DHPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHPS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHPS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 87252720
• NCBI GeneID: 330817
• NCBI Accession #: NP_001034603.1
• NCBI GenBank Nucleotide #: NM_001039514.1
• UniProt Accession #: Q3TXU5; E9QM48
• Molecular Weight: 40,642 Da
• NCBI Official Full Name: deoxyhypusine synthase
• NCBI Official Synonym Full Names: deoxyhypusine synthase
• NCBI Official Symbol: Dhps
• NCBI Official Synonym Symbols: Dhs
• NCBI Protein Information: deoxyhypusine synthase
• UniProt Protein Name: Deoxyhypusine synthase
• Protein Family: Deoxyhypusine synthase
• UniProt Gene Name: Dhps
• UniProt Synonym Gene Names: DHS

Uniprot Description

DHPS: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Belongs to the deoxyhypusine synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.5.1.46; Transferase

Chromosomal Location of Human Ortholog: 8|8 C3

Cellular Component: cytoplasm

Molecular Function: deoxyhypusine synthase activity; identical protein binding; transferase activity

Biological Process: glucose homeostasis; peptidyl-lysine modification to peptidyl-hypusine; positive regulation of T cell proliferation; protein homotetramerization; spermidine catabolic process; spermidine metabolic process