Diacylglycerol Kinase Eta Recombinant Protein | DGKh recombinant protein

Recombinant Diacylglycerol Kinase Eta (DGKh)

Cat. Num. AAA2123335

• Gene Names: DGKH; DGKeta
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Diacylglycerol Kinase Eta; Recombinant Diacylglycerol Kinase Eta (DGKh); Diglyceride kinase eta; DGKh recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Asp317~Thr451
• Sequence Length: 1164

• Applicable Applications for DGKh recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Secreted
• Isoelectric Point: 6.8
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for DGKh recombinant protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 325197213
• NCBI GeneID: 160851
• NCBI Accession #: NP_001191433.1
• NCBI GenBank Nucleotide #: NP_001191433.1
• UniProt Accession #: Q86XP1
• Molecular Weight: Predicted Molecular Mass: 18.6kDa; Accurate Molecular Mass: 16kDa
• NCBI Official Full Name: diacylglycerol kinase eta isoform 1
• NCBI Official Synonym Full Names: diacylglycerol kinase eta
• NCBI Official Symbol: DGKH
• NCBI Official Synonym Symbols: DGKeta
• NCBI Protein Information: diacylglycerol kinase eta
• UniProt Protein Name: Diacylglycerol kinase eta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKH
• UniProt Synonym Gene Names: DAG kinase eta; DGK-eta
• UniProt Entry Name: DGKH_HUMAN

NCBI Description

This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]

Uniprot Description

DGKH: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization. Belongs to the eukaryotic diacylglycerol kinase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, other; EC 2.7.1.107; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: nuclear membrane; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; protein oligomerization