Human Dengue Fever Antibody ELISA Kit | DF elisa kit

Human Dengue Fever Antibody ELISA Kit

Cat. Num. AAA5492

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Human
• Synonyms: Dengue Fever Antibody; Human Dengue Fever Antibody ELISA Kit; DF elisa kit

• Reactivity: Human
• Sequence Length: 228

• Samples: Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (?) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for DF elisa kit

Intended Uses: For the qualitative determination of activated Human Dengue Fever Antibody (DF) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.

Principle of the Assay||The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human DF in samples. Add sample to wells pre-coated with one Human Dengue Fever antigen, at same time add HRP-conjugated Human Dengue Fever secondy antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human DF in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

NCBI and Uniprot Product Information

• NCBI GI #: 49168634
• NCBI GeneID: 1675
• UniProt Accession #: Q6FHW3; P00746
• Molecular Weight: 24,423 Da
• NCBI Official Full Name: DF
• NCBI Official Synonym Full Names: complement factor D (adipsin)
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin)
• UniProt Protein Name: DF protein
• UniProt Gene Name: DF
• UniProt Entry Name: Q6FHW3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

Uniprot Description

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.46

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; blood coagulation; proteolysis; complement activation

Disease: Complement Factor D Deficiency