Testing Data
(Detection of pyroptosis in JurkatsJurkats were stained with Pyroptosis 660 Caspase-1 Kit for two hours followed by treatment with 10 ?M Nigericin as shown. Pyroptosis could be detected by an increase in active Caspase-1 detected by an increase in 660 far red fluorescence. Data analyzed on the ZE5 Cell Analyzer)
Caspase-1 Detection Kit | Casp1 detection kit
Pyroptosis 660 Caspase-1 Kit
Gene Names
Casp1; Ice; Il1bc
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
Caspase-1; Pyroptosis 660 Caspase-1 Kit; Pyroptosis 660 Caspase-1; Casp1 detection kit
Form/Format
660 (Red Fluorescence)
Sequence Length
402
Applicable Applications for Casp1 detection kit
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Preparation and Storage
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL.
Store the unopened kit (and each unopened component) according to the storage instructions on each component label. Store the Nigericin at -20 degree C.
Once reconstituted, the Nigericin stock should be used immediately or alliquoted and stored at -20 degree C for 12 months.
Avoid repeated freezing and thawing.
Store the unopened kit (and each unopened component) according to the storage instructions on each component label. Store the Nigericin at -20 degree C.
Once reconstituted, the Nigericin stock should be used immediately or alliquoted and stored at -20 degree C for 12 months.
Avoid repeated freezing and thawing.
Testing Data
(Detection of pyroptosis in JurkatsJurkats were stained with Pyroptosis 660 Caspase-1 Kit for two hours followed by treatment with 10 ?M Nigericin as shown. Pyroptosis could be detected by an increase in active Caspase-1 detected by an increase in 660 far red fluorescence. Data analyzed on the ZE5 Cell Analyzer)
Testing Data
(Detection of pyroptosis in JurkatsJurkats were stained with Pyroptosis 660 Caspase-1 Kit for two hours followed by treatment with 10 ?M Nigericin as shown. Pyroptosis could be detected by an increase in active Caspase-1 detected by an increase in 660 far red fluorescence. Data analyzed on the ZE5 Cell Analyzer)
Related Product Information for Casp1 detection kit
Description: Pyroptosis 660 Caspase-1 Kit utilizes the popular FLICA technology to detect caspase-1 activation. This kits contain the caspase-1 inhibitor reagent YVAD-FMK, which has the preferred binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD) (Chapman, 1992). This preferred caspase-1 binding sequence is labeled with 660 a far red fluorescent dye and linked to a fluoromethyl ketone (FMK) reactive entity. Caspase-1 will not cleave the FLICA inhibitor probe; instead, it forms an irreversible covalent bond with the FMK group on the reagent and becomes inhibited from further enzymatic activity.
Test Principle: To use FLICA, add directly to the cell culture medium, incubate, and wash. FLICA is cell-permeant and will efficiently diffuse in and out of all cells. If there is an active caspase-1 enzyme inside the cell, it will covalently bind with YVAD-FMK and retain the fluorescent signal within the cell. Unbound FLICA will diffuse out of the cell during the subsequent wash steps. Therefore, positive cells will retain a higher concentration of FLICA and fluoresce brighter than negative cells. There is no interference from pro-caspases or inactive forms of the enzymes. After labeling with FLICA, cells can be counter-stained with other reagents and fixed or frozen. Cells labeled with YVAD-FMK can be counter-stained with reagents such as the red live/dead stains Propidium Iodide and 7-AAD. Nuclear morphology may be concurrently observed using Hoechst 33342 (included in the kit), a blue DNA-binding dye. Cells can be viewed through a fluorescence microscope or flow cytometer.
Test Principle: To use FLICA, add directly to the cell culture medium, incubate, and wash. FLICA is cell-permeant and will efficiently diffuse in and out of all cells. If there is an active caspase-1 enzyme inside the cell, it will covalently bind with YVAD-FMK and retain the fluorescent signal within the cell. Unbound FLICA will diffuse out of the cell during the subsequent wash steps. Therefore, positive cells will retain a higher concentration of FLICA and fluoresce brighter than negative cells. There is no interference from pro-caspases or inactive forms of the enzymes. After labeling with FLICA, cells can be counter-stained with other reagents and fixed or frozen. Cells labeled with YVAD-FMK can be counter-stained with reagents such as the red live/dead stains Propidium Iodide and 7-AAD. Nuclear morphology may be concurrently observed using Hoechst 33342 (included in the kit), a blue DNA-binding dye. Cells can be viewed through a fluorescence microscope or flow cytometer.
Product Categories/Family for Casp1 detection kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
caspase-1
NCBI Official Synonym Full Names
caspase 1
NCBI Official Symbol
Casp1
NCBI Official Synonym Symbols
Ice; Il1bc
NCBI Protein Information
caspase-1
Protein Family
NCBI Description
apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
Research Articles on Casp1
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Product Notes
The Casp1 (Catalog #AAA225841) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Caspase-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the Casp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-1, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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