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Typical Testing Data/Standard Curve (for reference only)

Bovine Beta-defensin 4 ELISA Kit | DEFB4 elisa kit

Bovine Beta-defensin 4 ELISA Kit

Gene Names
DEFB4A; BNBD4; DEFB4; BNBD-4
Reactivity
Bovine
Synonyms
Beta-defensin 4; Bovine Beta-defensin 4 ELISA Kit; DEFB4 elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Specificity
This assay recognizes recombinant and natural bovine Beta-defensin 4. No significant cross-reactivity or interference was observed.
Sequence Length
72
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Detection Range
0.31 ng/ml -20.0 ng/ml.
Intended Uses
This immunoassay kit allows for the in vitro quantitative determination of bovine Beta-defensin 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DEFB4 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-defensin 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-defensin 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-defensin 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Beta-defensin 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7,161 Da
NCBI Official Full Name
beta-defensin 4
NCBI Official Symbol
DEFB4A
NCBI Official Synonym Symbols
BNBD4; DEFB4; BNBD-4
NCBI Protein Information
beta-defensin 4
UniProt Protein Name
Beta-defensin 4
Protein Family
UniProt Gene Name
DEFB4
UniProt Synonym Gene Names
BNBD4
UniProt Entry Name
DEFB4_BOVIN

Uniprot Description

Has bactericidal activity. Active against E.coli ML35 and S.aureus 502A.

Research Articles on DEFB4

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Product Notes

The Bovine DEFB4 defb4 (Catalog #AAA288127) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288127 ELISA Kit recognizes Bovine DEFB4. It is sometimes possible for the material contained within the vial of "Beta-defensin 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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