BD3 Recombinant Protein | Defb3 recombinant protein
BD3, Recombinant, Human (beta Defensin-3)
Gene Names
Defb3; BD-3; MGC129397
Purity
Highly Purified95% as determined by RP-HPLC and SDS-PAGE. Purified by proprietary chromatographic techniques.
Synonyms
BD3; Recombinant; Human (beta Defensin-3); Defb3 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by RP-HPLC and SDS-PAGE. Purified by proprietary chromatographic techniques.
95% as determined by RP-HPLC and SDS-PAGE. Purified by proprietary chromatographic techniques.
Form/Format
Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH20 to >0.1mg/ml.
Sequence
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Defb3 recombinant protein
Recombinant Human BD-3 produced in E.coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5.16kD.
BBeta-Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds. The 45 aa mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively. It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression. In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S. BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast. BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability. Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties. BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4, and with HSV glycoprotein B plus its receptor heparan sulfate, and by forming a protective coating on the surface of influenza virus target cells.
BBeta-Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds. The 45 aa mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively. It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression. In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S. BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast. BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability. Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties. BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4, and with HSV glycoprotein B plus its receptor heparan sulfate, and by forming a protective coating on the surface of influenza virus target cells.
Product Categories/Family for Defb3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5.16kD
NCBI Official Full Name
beta-defensin 3
NCBI Official Synonym Full Names
defensin beta 3
NCBI Official Symbol
Defb3
NCBI Official Synonym Symbols
BD-3; MGC129397
NCBI Protein Information
beta-defensin 3; OTTMUSP00000022575
UniProt Protein Name
Beta-defensin 3
Protein Family
UniProt Gene Name
Defb3
UniProt Synonym Gene Names
Bd3
UniProt Entry Name
DEFB3_MOUSE
Uniprot Description
Function: Antimicrobial activity against Gram-negative bacteria E.coli and P.aeruginosa. Ref.1
Subcellular location: Secreted.
Tissue specificity: Highest expression in salivary glands, epididymis, ovary and pancreas and to a lesser extent in lung, liver and brain. Low or no expression in skeletal muscle and tongue. Ref.1 Ref.3
Induction: By bacterial infection. Ref.1
Sequence similarities: Belongs to the beta-defensin family. LAP/TAP subfamily.
Research Articles on Defb3
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Product Notes
The Defb3 defb3 (Catalog #AAA650059) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK. It is sometimes possible for the material contained within the vial of "BD3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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