TGFBR3 recombinant protein

TGFBR3 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122810

• Gene Names: Ddr2; Ntrkr3; tyro10; AW495251
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TGFBR3; TGFBR3 Protein; Mouse; Recombinant (His Tag); Mouse TGFBR3/Betaglycan Protein (His Tag); 1110036H20Rik Protein; AU015626 Protein; AW215636 Protein; TBRIII Protein; transforming growth factor; beta receptor III; TGFBR3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg399

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse TGFBR3 (NP_072075.2)(Met 1-Arg 399) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TGFBR3 recombinant protein

Background: Betaglycan also known as transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate/heparan sulfate proteoglycan. TGFBR3 is a transforming growth factor (TGF)-beta type III receptor. This receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is the TGF-beta component most commonly downregulated among localized human prostate cancer studies. TGFBR3 knockdown led to focus formation and enhanced expression of CD133, a marker found on prostate cancer stem cells. TGFBR3 is an accessory receptor that binds to and modulates the activities of both transforming growth factor-beta (TGFbeta) and inhibin, two members of the TGFbeta superfamily of growth factors that regulate many aspects of reproductive biology. TGFBR3 is known to be expressed in adult testis and ovary, but little is known about this receptor during gonadogenesis.

References

Johnson DW, et al. (1996) Assignment of human transforming growth factor-beta type I and type III receptor genes (TGFBR1 and TGFBR3) to 9q33-q34 and 1p32-p33, respectively. Genomics. 28 (2): 356-7.Rotzer D, et al. (2001) Type III TGF-beta receptor-independent signalling of TGF-beta2 via T betaRII-B, an alternatively spliced TGF- type II receptor. EMBO J. 20 (3): 480-90.Gao J, et al. (1999) Expression of transforming growth factor-beta receptors types II and III within various cells in the rat periodontium. J Periodont Res. 34 (2): 113-22.

NCBI and Uniprot Product Information

• NCBI GI #: 158508514
• NCBI GeneID: 18214
• NCBI Accession #: NP_072075.2
• NCBI GenBank Nucleotide #: NM_022563.2
• UniProt Accession #: Q62371; B2RSD7
• Molecular Weight: 96,482 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor family, member 2
• NCBI Official Symbol: Ddr2
• NCBI Official Synonym Symbols: Ntrkr3; tyro10; AW495251
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Transforming growth factor
• UniProt Gene Name: Ddr2
• UniProt Synonym Gene Names: Ntrkr3; Tkt; Tyro10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_MOUSE

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; DDR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; collagen binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: ossification; collagen fibril organization; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of osteoblast differentiation; positive regulation of fibroblast proliferation; biomineral formation; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription factor activity; phosphorylation; regulation of bone mineralization; transmembrane receptor protein tyrosine kinase signaling pathway