Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) Recombinant Protein | DDAH2 recombinant protein

Recombinant Dimethylarginine Dimethylaminohydrolase 2 (DDAH2)

Cat. Num. AAA2010861

• Gene Names: DDAH2; G6a; DDAH; NG30; DDAHII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Dimethylarginine Dimethylaminohydrolase 2 (DDAH2); Recombinant Dimethylarginine Dimethylaminohydrolase 2 (DDAH2); DDAH2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AGLPALDLAK AQREHGVLGG KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ KLSDVTLVPV SCSEL
• Sequence Length: 285

• Applicable Applications for DDAH2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala31~Leu265 (Accession # O95865) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 7524354
• NCBI GeneID: 23564
• NCBI Accession #: NP_039268.1
• NCBI GenBank Nucleotide #: NM_013974.1
• UniProt Accession #: O95865; A2BEZ7
• Molecular Weight: 28.6kDa
• NCBI Official Full Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
• NCBI Official Synonym Full Names: dimethylarginine dimethylaminohydrolase 2
• NCBI Official Symbol: DDAH2
• NCBI Official Synonym Symbols: G6a; DDAH; NG30; DDAHII
• NCBI Protein Information: N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; DDAH-2; protein G6a; S-phase protein; dimethylargininase-2; alternative name: NG30, G6a; dimethylarginine dimethylaminohydrolase II; NG-dimethylarginine dimethylamino hydrolase homolog
• UniProt Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
• Protein Family: N(G),N(G)-dimethylarginine dimethylaminohydrolase
• UniProt Gene Name: DDAH2
• UniProt Synonym Gene Names: DDAH; G6A; NG30; DDAH-2
• UniProt Entry Name: DDAH2_HUMAN

Uniprot Description

DDAH2: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family.

Protein type: EC 3.5.3.18; Apoptosis; Hydrolase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: centrosome; mitochondrion; cytoplasm

Molecular Function: amino acid binding; protein binding; dimethylargininase activity; catalytic activity

Biological Process: positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; arginine catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; negative regulation of apoptosis