ATP-dependent DNA helicase dda (dda) Recombinant Protein | dda recombinant protein

Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda)

Cat. Num. AAA7136989

• Gene Names: dda; sud
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: ATP-dependent DNA helicase dda (dda); Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda); dda recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process); If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyop
• Sequence Positions: 1-439aa, Full Length
• Sequence: MTFDDLTEGQKNAFNIVMKAIKEKKHHVTINGPAGTGKTTLTKFIIEALISTGGTGIILAAPTHAAKKILSKLSGKEASTIHSILKINPVTYEENVLFEQKEVPDLAKCRVLICDEVSMYDRKLFKILLSTIPPWCTIIGIGDNKQIRPVEPGENTAYISPFFTHKDFYQCELTEVKRSNAPIIDVATDVRNGKWNYDKVVDGHGVRGFTGDTALRDFMVNYFSIVKSLDDLFENRVMAFTNKSVDKLNSIIRKKIFETDKDFIVGEIIVMQEPLFKTYKIDGKPVSEIIFNNGQLVRIIEAEYTSTFVKARGVPGEYLIRHWDLTVETYGDDEYYREKIKIISSDEELYKFNLFLAKTAETYKNWNKGGKAPWSDFWDAKSQFSKVKALPASTFHKAQGMSVDRAFIYTPCIHYADVELAQQLLYVGVTRGRYDVFYV

• Species: Enterobacteria phage T4 (Bacteriophage T4)
• Tag: N-terminal 6xHis-tagged
• Function: DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Product Categories/Family for dda recombinant protein

Neuroscience

References

"Overexpression, purification, sequence analysis, and characterization of the T4 bacteriophage dda DNA helicase."Hacker K.J., Alberts B.M.J. Biol. Chem. 267:20674-20681 (1992)

NCBI and Uniprot Product Information

• NCBI GI #: 9632724
• NCBI GeneID: 1258784
• NCBI Accession #: NP_049632.1
• NCBI GenBank Nucleotide #: NC_000866.4
• UniProt Accession #: P32270; Q9T0W1
• Molecular Weight: 49,897 Da
• NCBI Official Full Name: Dda DNA helicase
• NCBI Official Symbol: dda
• NCBI Official Synonym Symbols: sud
• NCBI Protein Information: DNA-dependent ATPase, DNA helicase
• UniProt Protein Name: ATP-dependent DNA helicase dda
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: dda
• UniProt Synonym Gene Names: sud

Uniprot Description

DNA helicase that stimulates viral DNA replication and recombination. Plays a role in T4 DNA replication initiation by selecting and activating DNA origins. Acts by dissociating and reassociating with the DNA molecule being unwound. Unwinds DNA as a monomer in a 5'-to-3' direction at a rate of 250 bp/s and can efficiently displace proteins from the DNA.