Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Bovine Doublecortin ELISA Kit | DCX elisa kit

Bovine Doublecortin ELISA Kit

Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Bovine
Synonyms
Doublecortin; Bovine Doublecortin ELISA Kit; DCX elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Intended Uses
This DCX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DCX. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This DCX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DCX. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DCX elisa kit
Principle of the Assay: DCX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DCX antibody and an DCX-HRP conjugate. The assay sample and buffer are incubated together with DCX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCX concentration since DCX from samples and DCX-HRP conjugate compete for the anti-DCX antibody binding site. Since the number of sites is limited, as more sites are occupied by DCX from the sample, fewer sites are left to bind DCX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCX concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DCX elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
40,215 Da
NCBI Official Full Name
doublecortin
NCBI Official Synonym Full Names
doublecortin
NCBI Official Symbol
DCX
NCBI Official Synonym Symbols
DC; DBCN; LISX; SCLH; XLIS
NCBI Protein Information
neuronal migration protein doublecortin; lis-X; doublin; doublecortex; lissencephalin-X
UniProt Protein Name
Neuronal migration protein doublecortin
UniProt Gene Name
DCX
UniProt Synonym Gene Names
DBCN; LISX; Lis-X
UniProt Entry Name
DCX_HUMAN

NCBI Description

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

Doublecortin: Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Interacts with tubulin. Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: microtubule; neuron projection; microtubule associated complex; cytoskeleton; cytosol

Molecular Function: protein binding; microtubule binding; protein kinase binding

Biological Process: nervous system development; axon guidance; central nervous system development; axon extension; dendrite morphogenesis; neuron migration; central nervous system projection neuron axonogenesis; brain development

Disease: Lissencephaly, X-linked, 1

Research Articles on DCX

Similar Products

Product Notes

The Bovine DCX dcx (Catalog #AAA742038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742038 ELISA Kit recognizes Bovine DCX. It is sometimes possible for the material contained within the vial of "Doublecortin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.