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SDS-Page

Dynactin 2 (DCTN2) Recombinant Protein | DCTN2 recombinant protein

Recombinant Dynactin 2 (DCTN2)

Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Dynactin 2 (DCTN2); Recombinant Dynactin 2 (DCTN2); P50; DCTN50; RBP50; 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; p50 dynamitin; DCTN2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Freeze-dried powder
Sequence Length
403
Applicable Applications for DCTN2 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Fragment
Ala2~Lys401
Tag
N-terminal His-tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Source Note
Prokaryotic expression
Organism Species
Human
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
5.1
Subcellular Location
Cytoplasm, Cytoskeleton, microtubule organizing center, centrosome.
Usage
Reconstitute in PBS or others.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page
Product Categories/Family for DCTN2 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47.8kDa
NCBI Official Full Name
dynactin subunit 2 isoform 2
UniProt Protein Name
Dynactin subunit 2
Protein Family
UniProt Gene Name
DCTN2
UniProt Synonym Gene Names
DCTN50; DCTN-50

Uniprot Description

dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytoskeletal; Microtubule-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: centrosome; cytoplasm; cytosol; dynactin complex; kinetochore; vesicle

Molecular Function: identical protein binding; protein binding; spectrin binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell proliferation; ER to Golgi vesicle-mediated transport; G2/M transition of mitotic cell cycle; mitosis; mitotic spindle organization and biogenesis

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Product Notes

The DCTN2 dctn2 (Catalog #AAA2086822) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dynactin 2 (DCTN2) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the DCTN2 dctn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dynactin 2 (DCTN2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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