Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
DAPK2 recombinant protein
DAPK2, Active
Applications
Western Blot
Synonyms
DAPK2; Active; DRP-1; MGC119312; DAPK2 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for DAPK2 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DAPK2 recombinant protein
Recombinant human full-length DAPK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST The gene accession number is NM_014326.
DAPK2 or death-associated protein kinase 2 belongs to a family of proapoptotic Ca(2+)/calmodulin-regulated serine/threonine kinases. Overexpression of DAPK2 induces cell apoptosis. DAPK2 has been shown to be a novel Sp1-dependent target gene for E2F1 and Krüppel-like factor 6 (KLF6) in cell death response (1). Both E2F1 and KLF6 strongly activate the DAPK2 promoter. DAPK2 plays a role in granulopoiesis where it is highly expressed. beta-catenin can block anoikis of malignant kidney and intestinal epithelial cells and promote their anchorage-independent growth by down-regulating DAPK2 (2). beta-catenin-induced down-regulation of DAPK2 requires the presence of the transcription factor TCF-4.
DAPK2 or death-associated protein kinase 2 belongs to a family of proapoptotic Ca(2+)/calmodulin-regulated serine/threonine kinases. Overexpression of DAPK2 induces cell apoptosis. DAPK2 has been shown to be a novel Sp1-dependent target gene for E2F1 and Krüppel-like factor 6 (KLF6) in cell death response (1). Both E2F1 and KLF6 strongly activate the DAPK2 promoter. DAPK2 plays a role in granulopoiesis where it is highly expressed. beta-catenin can block anoikis of malignant kidney and intestinal epithelial cells and promote their anchorage-independent growth by down-regulating DAPK2 (2). beta-catenin-induced down-regulation of DAPK2 requires the presence of the transcription factor TCF-4.
Product Categories/Family for DAPK2 recombinant protein
References
1.Britschgi A. et al: DAPK2 is a novel E2F1/KLF6 target gene involved in their proapoptotic function. Oncogene. 2008 Sep 25;27(43):5706-16.
2.Li, H. et al: Down-regulation of death-associated protein kinase-2 is required for beta-catenin-induced anoikis resistance of malignant epithelial cells. J Biol Chem. 2009 Jan 23;284(4):2012-22.
2.Li, H. et al: Down-regulation of death-associated protein kinase-2 is required for beta-catenin-induced anoikis resistance of malignant epithelial cells. J Biol Chem. 2009 Jan 23;284(4):2012-22.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~67kDa
Protein Family
Similar Products
Product Notes
The DAPK2 (Catalog #AAA516772) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DAPK2 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the DAPK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPK2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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